ICSI - Intracytoplasmic Sperm Injection

If a couple is treated for male factor infertility (low count of sperm present in ejaculate, abnormal morphology or motility) or other immunological or so-called unclear (idiopathic) reasons or if in previous cycle fertilization failed, the technique of ICSI (intracytoplasmic sperm injection) is used to fertilize the eggs.

For the purpose of oocyte fertilization, one sperm is aspirated inside a tiny glass pipette and then injected inside the ovum. After standard culture embryos are either ready for transfer or cryo-conservation.